Some colleagues of mine are running an Illumina TSCA panel on FFPE samples using the MiSeq, they have just starting using a water blank and putting it through the whole process including sequencing on the MiSeq. As a diagnostic lab using a water blank is common practise for every other technique we use.
They were surprised at how many reads were assigned to the blank. I was just wondering if anyone else had tried running this sort of blank on a MiSeq or any other NGS machine for that matter. Or if anyone has any thoughts on using water blanks on NGS?
Thanks in advance!!
They were surprised at how many reads were assigned to the blank. I was just wondering if anyone else had tried running this sort of blank on a MiSeq or any other NGS machine for that matter. Or if anyone has any thoughts on using water blanks on NGS?
Thanks in advance!!
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