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Hello,
TruesmallRNASeq kit is able to clone and sequence both strands of the small RNA?
Thanks
TruesmallRNASeq kit is able to clone and sequence both strands of the small RNA?
Thanks
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Last edited by Schelarina; 05-16-2014, 04:13 AM. -
Small RNAs, like the majority of RNA species are single stranded, unless I am misinterpreting your meaning of "both strands". -
small RNAs are first processed as double stranded RNA and then dissociate and the sense strand is ligation competent. I am not 100% if the antisense strand which has P group at 3'end can also be ligated, that s why I ve asked..
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Ligation reaction occurs between 3' OH of one nucleic acid strand with 5' phosphate of another strand. 3' P group would inhibit ligation and amplification.Comment
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