Hi, everyone,
I made four libraries this week. But today when I ran a DNA gel, I found that the concentrations (DNA band intensity) of the four libraries have great difference. The concentration of the highest one is about 3 times of the lowest one. I am supposed to compare gene expression level changes among these four libraries. I wonder whether the library concentrations difference will affect RNA-sequencing results. For example, some reads may overpresent the library. Does anyone have similar situation?
Thanks!
I made four libraries this week. But today when I ran a DNA gel, I found that the concentrations (DNA band intensity) of the four libraries have great difference. The concentration of the highest one is about 3 times of the lowest one. I am supposed to compare gene expression level changes among these four libraries. I wonder whether the library concentrations difference will affect RNA-sequencing results. For example, some reads may overpresent the library. Does anyone have similar situation?
Thanks!
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