We are using the MiSeq machine (2*250, V2 kit) for a Truseq library sequencing. As we find the cluster density is low (only 239k) on the cycle 3, can we just stop the machine now and add additional libraries and re-run the protocol?
Because the MiSeq machine used to report an error just after the cluster generation step. We washed and restarted the machine, and all process worked fine. I just wondering if I can do that again on cycle 3.
add:
The run continue and yield 2.7G result at last. The normal run with density 800K should yield 7.5G data.
add:
We quantified the libraries with real-time PCR using KAPA kit.
Because the MiSeq machine used to report an error just after the cluster generation step. We washed and restarted the machine, and all process worked fine. I just wondering if I can do that again on cycle 3.
add:
The run continue and yield 2.7G result at last. The normal run with density 800K should yield 7.5G data.
add:
We quantified the libraries with real-time PCR using KAPA kit.
Comment