Tweet
Hello,
Is it possible to do a run of 60 cycles with a 50 cycles MiSeq Reagent Kit v2 ?
Is it possible to do a run of 60 cycles with a 50 cycles MiSeq Reagent Kit v2 ?
-
-
Hi jsvinco,
Each kit is loaded with 25 additional cycles for indexing. So unless you need both indexing reads the answer is a 'yes'. If you need both indexing reads as well as the 50 cycles you might be pushing it - we have pushed the machine 5 cycles over the total cycle count (i.e 330 cycles on a 300 kit) and it behaved (although the machine complains at set-up). Other might have gone further?
Hope this helps - Cheers, Mike. -
Thank you for your help. It's very helpful to now that.
jsvincoComment
-
Innovations in next-generation sequencing technologies and techniques are driving more precise and comprehensive exploration of complex biological systems. Current advancements include improved accessibility for long-read sequencing and significant progress in single-cell and 3D genomics. This article explores some of the most impactful developments in the field over the past year.
Long-Read Sequencing
Long-read sequencing has...12-02-2024, 01:49 PM -
The field of immunogenetics explores how genetic variations influence immune responses and susceptibility to disease. In a recent SEQanswers webinar, Oscar Rodriguez, Ph.D., Postdoctoral Researcher at the University of Louisville, and Ruben MartÃnez Barricarte, Ph.D., Assistant Professor of Medicine at Vanderbilt University, shared recent advancements in immunogenetics. This article discusses their research on genetic variation in antibody loci, antibody production processes,...11-06-2024, 07:24 PM
Comment