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Increase the number of cycle with a 50 cycles MiSeq Reagent Kit v2

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  • Increase the number of cycle with a 50 cycles MiSeq Reagent Kit v2

    Hello,

    Is it possible to do a run of 60 cycles with a 50 cycles MiSeq Reagent Kit v2 ?

  • #2
    Hi jsvinco,
    Each kit is loaded with 25 additional cycles for indexing. So unless you need both indexing reads the answer is a 'yes'. If you need both indexing reads as well as the 50 cycles you might be pushing it - we have pushed the machine 5 cycles over the total cycle count (i.e 330 cycles on a 300 kit) and it behaved (although the machine complains at set-up). Other might have gone further?

    Hope this helps - Cheers, Mike.

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    • #3
      Thank you for your help. It's very helpful to now that.

      jsvinco

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