Paired end generation

[HICORE]

When are the paired ends generated? During the Cluster generation step or during the sequencing step?

The fact that you need twice as sequencing reagents for paired end runs than single ends makes me think that it is during the sequencing step

Thanks for your help!

[ECO]

You capture both ends of a longer molecule through some molecular biology trickery...so it's in the library prep. Then there are two sets of sequencing reads generated from either end of the molecule.

This is pretty basic and should be found from any illumina tech bulletin or manual on their website. =]