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  • Difference when shearing with Low-EDTA and Water?

    Dear all,
    Does anyone have ever test the shearing protocol provided by Covaris with different buffer? e.g. low-EDTA buffer and Water?
    thank you!
    Wei

  • #2
    TE buffer which has been recommended is the best option. For fragmentation to small sizes 250bp< degradation level increases if water is used for resuspension of DNA.

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    • #3
      I haven't compared all different buffer types but we routinely use TE - low EDTA (10 mM Tris-HCl, pH 8.0, 0.1 mM EDTA) with excellent results, fragmenting to ~160bp.

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