Difference when shearing with Low-EDTA and Water?

[wangwde]

Dear all,
Does anyone have ever test the shearing protocol provided by Covaris with different buffer? e.g. low-EDTA buffer and Water?
thank you!
Wei

[nucacidhunter]

TE buffer which has been recommended is the best option. For fragmentation to small sizes 250bp< degradation level increases if water is used for resuspension of DNA.

[neurula]

I haven't compared all different buffer types but we routinely use TE - low EDTA (10 mM Tris-HCl, pH 8.0, 0.1 mM EDTA) with excellent results, fragmenting to ~160bp.