Hi All,
I'm new to this whole NGS business. I will be using an Illumina GAII. In a recent article from Bio Techniques, I read that longer (about 600bp) fragments can increase the uniformity of coverage across a targeted region. In the paper, amplicons were fragmented to 200bp prior to adaptor ligation and subsequence sequencing. Does anyone know why this would increase uniformity? Also, does anyone know if it is possible to successfully generate clusters during bridge amplification with fragments larger than 200-300 bp? And if so, how large?
I'm new to this whole NGS business. I will be using an Illumina GAII. In a recent article from Bio Techniques, I read that longer (about 600bp) fragments can increase the uniformity of coverage across a targeted region. In the paper, amplicons were fragmented to 200bp prior to adaptor ligation and subsequence sequencing. Does anyone know why this would increase uniformity? Also, does anyone know if it is possible to successfully generate clusters during bridge amplification with fragments larger than 200-300 bp? And if so, how large?
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