Tweet
Hello,
We plan an RNAseq analysis to study gene expression in a bacteria that we have an in house reference genome assembly (~7.8Mbp) and gene annotation (7000 genes) that we are very confident about (both).
in a previous RNAseq analysis we used 100bp SE reads with ~3M reads per sample (after QC) and got nice coverage and statistics for our gene expression study.
This time we will use a different service provider (due to some bureaucracy and budget issues) and they offered either ~7-8M reads of 80bp SE, or a similar number of reads but PE with 40bp length and around 350bp insert (for the same price).
Can anyone suggest me which offer to take ?
Thank you !
We plan an RNAseq analysis to study gene expression in a bacteria that we have an in house reference genome assembly (~7.8Mbp) and gene annotation (7000 genes) that we are very confident about (both).
in a previous RNAseq analysis we used 100bp SE reads with ~3M reads per sample (after QC) and got nice coverage and statistics for our gene expression study.
This time we will use a different service provider (due to some bureaucracy and budget issues) and they offered either ~7-8M reads of 80bp SE, or a similar number of reads but PE with 40bp length and around 350bp insert (for the same price).
Can anyone suggest me which offer to take ?
Thank you !
Comment