Hello, we are trying to quantify the expresion of human EB's vs human ES's RNA levels and splice variants for this we are doing a RNA Seq of both cell lines using 100bp PE. We've never done RNA seq before, how many lanes per sample do you need to get a good coverage so that we can tell if one splice variant is more frequent in one cell line than another???
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I am not yet an expert on this, but based on the amount of sequence being generated by HiSeq 2000 and the size of the transcriptome, I'm guessing one lane per sample. Anyone know for sure?Mendelian Disorder: A blogshare of random useful information for general public consumption. [Blog]
Breakway: A Program to Identify Structural Variations in Genomic Data [Website] [Forum Post]
Projects: U87MG whole genome sequence [Website] [Paper]
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Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.
We asked six companies the same four questions to learn about their latest products. The new technologies bring a lot to the table, including rethinking sequencing...
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