Hello, we are trying to quantify the expresion of human EB's vs human ES's RNA levels and splice variants for this we are doing a RNA Seq of both cell lines using 100bp PE. We've never done RNA seq before, how many lanes per sample do you need to get a good coverage so that we can tell if one splice variant is more frequent in one cell line than another???
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I am not yet an expert on this, but based on the amount of sequence being generated by HiSeq 2000 and the size of the transcriptome, I'm guessing one lane per sample. Anyone know for sure?Mendelian Disorder: A blogshare of random useful information for general public consumption. [Blog]
Breakway: A Program to Identify Structural Variations in Genomic Data [Website] [Forum Post]
Projects: U87MG whole genome sequence [Website] [Paper]
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Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?
Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating...10-14-2026, 09:55 AM
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