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Hello, we are trying to quantify the expresion of human EB's vs human ES's RNA levels and splice variants for this we are doing a RNA Seq of both cell lines using 100bp PE. We've never done RNA seq before, how many lanes per sample do you need to get a good coverage so that we can tell if one splice variant is more frequent in one cell line than another???
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I am not yet an expert on this, but based on the amount of sequence being generated by HiSeq 2000 and the size of the transcriptome, I'm guessing one lane per sample. Anyone know for sure?Mendelian Disorder: A blogshare of random useful information for general public consumption. [Blog]
Breakway: A Program to Identify Structural Variations in Genomic Data [Website] [Forum Post]
Projects: U87MG whole genome sequence [Website] [Paper]
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The sequencing world is rapidly changing due to declining costs, enhanced accuracies, and the advent of newer, cutting-edge instruments. Equally important to these developments are improvements in sequencing analysis, a process that converts vast amounts of raw data into a comprehensible and meaningful form. This complex task requires expertise and the right analysis tools. In this article, we highlight the progress and innovation in sequencing analysis by reviewing several of the...05-06-2024, 07:48 AM -
The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...04-22-2024, 07:01 AM
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