Hi! Is the flow cell analyzed in one go per base (as a whole), or scanned one part of the flow cell at a time, until all bases have been identified? How many cameras are there in llumina's instruments to image the flow cell? Thanks in advance!
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As far as I understand, the MiSeq, HiSeq 2500, and HiSeq 4000 each have a single camera. The flow cell is divided into lanes, swaths (left/right/center depending on the machine), surfaces (top and bottom), and tiles. A single tile is imaged at a time, except during focusing. The camera moves up a swath, imaging each tile's top surface. It does all the swaths for the top surface, then goes back and does the bottom surface for each tile.
The NextSeq is slightly different, with six cameras/sensors working as a single module, simultaneously imaging six different tiles.
I can't speak to the NovaSeq or iSeq. -
Thanks
Thanks a lot for the good answer, it helped a lot!Comment
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I do know Hiseq 2500's have two cameras. Here's a picture of the optical deck in case anyone is interested. You can see the green and red lasers at the left and top and the two cameras at angles on the right.
Click for bigger version.
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The immune system’s power comes from its genetic diversity, allowing myriad threats to be neutralized through first recognizing foreign antigens. That diversity is also what makes the immune system so difficult to study. Recent advances in sequencing technology and computational biology, however, are giving researchers new tools to understand immune responses and immune-related diseases in greater detail.
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