Hi! Is the flow cell analyzed in one go per base (as a whole), or scanned one part of the flow cell at a time, until all bases have been identified? How many cameras are there in llumina's instruments to image the flow cell? Thanks in advance!
-
-
As far as I understand, the MiSeq, HiSeq 2500, and HiSeq 4000 each have a single camera. The flow cell is divided into lanes, swaths (left/right/center depending on the machine), surfaces (top and bottom), and tiles. A single tile is imaged at a time, except during focusing. The camera moves up a swath, imaging each tile's top surface. It does all the swaths for the top surface, then goes back and does the bottom surface for each tile.
The NextSeq is slightly different, with six cameras/sensors working as a single module, simultaneously imaging six different tiles.
I can't speak to the NovaSeq or iSeq. -
Thanks
Thanks a lot for the good answer, it helped a lot!Comment
-
I do know Hiseq 2500's have two cameras. Here's a picture of the optical deck in case anyone is interested. You can see the green and red lasers at the left and top and the two cameras at angles on the right.
Click for bigger version.
Comment
-
Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?
Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating...10-14-2026, 09:55 AM -
Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.
We asked six companies the same four questions to learn about their latest products. The new technologies bring a lot to the table, including rethinking sequencing...
Comment