Hi,
We were preparing library for NextSeq whole exome sequencing. Our starting concentration before library preparation for three samples were 27ng/µL, 37ng/µL and 42ng/µL. We pooled library and when we checked our fragment size before running on sequencer by Bioanalyzer, we saw fragment size of 239 base pair which is smaller than expected. I want to know on which step we are making mistake which resulted in smaller insert size than expected?
We were preparing library for NextSeq whole exome sequencing. Our starting concentration before library preparation for three samples were 27ng/µL, 37ng/µL and 42ng/µL. We pooled library and when we checked our fragment size before running on sequencer by Bioanalyzer, we saw fragment size of 239 base pair which is smaller than expected. I want to know on which step we are making mistake which resulted in smaller insert size than expected?
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