Does anyone know the composition of the Tagment Wash Buffer in the Nextera DNA Flex Library Prep (20018704) protocol? Does it contain alcohol? PEG?
TIA
TIA
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I would check out the SMART-SEQ2 protocol. They did homebrew something similar. I vaguely remember SDS to denature the enzyme. -
Hi BexMultiplex,
According to the Nextera Flex patent, TWB is:
-100 mM Tris HCl pH 8.5
-100 mM NaCL
-0.1% Tween 20
We have a Tecan, and are going to be reducing RXN volumes down to 1/4, and at that point TWB becomes a limiting reagent. I will be trying my own eventually, but I have not used it yet personally myself, so i can't promise it will work
If you are ever trying to find what a reagent is, looking at the patent (if you can find it) is probably the best way to get this kind of information. It also really helpful for understanding how a kit work when the protocol is vague.
Hope this helps!Comment
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Thanks @UCan'tBcereus, that's really helpful!Comment
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