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I'm using unique dual index primer pairs for my experiments. I'm multiplexing around 12 samples per NextSeq run. I don't know how to create a custom library in BaseSpace that will allow me to assign the unique primer pairs to all my samples as it's limited by a 12 x 8 format (12- i7 indexes and 8-i5 indexes, I need to be able to enter 12 i5 indexes). Any help would be greatly appreciated.
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I ran into a similar issue recently. If you have the Dx version of the NextSeq, there is a software update that can do FASTQ generation on the machine similar to the MiSeq.
The other option is a work around where you will have to create two custom library kit. Each kit can do 8 UDI samples if you set your samples in a diagonal so that each row and column will have an unique index. After that just pool them together in the pool tab in BaseSpace. -
I don't have the Dx version but your workaround will be just fine, I didn't think it would let me combine samples from two different "kits". Thanks for the help!Comment
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custom library
https://support.illumina.com/bulleti...sequence-.html
Here's the bulletin from Illumina describing how to create a custom library prep kit in BaseSpace.Comment
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The first FDA-approved CRISPR-based therapy marked the transition of therapeutic gene editing from a dream to reality1. CRISPR technologies have streamlined gene editing, and CRISPR screens have become an important approach for identifying genes involved in disease processes2. This technique introduces targeted mutations across numerous genes, enabling large-scale identification of gene functions, interactions, and pathways3. Identifying the full range...08-27-2024, 04:44 AM
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