Hi. I have some samples from another lab that were prepped with the Qiagen kit. All I have is a limited amount of DNA, & the DNA is just okay quality. I did a clean up and ran them on the Miseq. Some of the sequencing quality is poor. Any recommendations for improving the data quality on the analysis side? Or even something to do if I redo the library prep? Thanks! The samples I prepped ran fine, so it isn’t a run issue.
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Could you let us know what specific problems you see with the data (and the DNA samples?)? -
What library prep tools are at your disposal? I'd suggest Nextera XT for low-concentration samples. You could also reamplify your libraries prior to normalization. If your samples are low on concentration, try vacuum concentration.Comment
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NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification,...10-30-2026, 06:46 AM
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