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What library prep tools are at your disposal? I'd suggest Nextera XT for low-concentration samples. You could also reamplify your libraries prior to normalization. If your samples are low on concentration, try vacuum concentration. -
Could you let us know what specific problems you see with the data (and the DNA samples?)?Leave a comment:
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Poor DNA prep
Hi. I have some samples from another lab that were prepped with the Qiagen kit. All I have is a limited amount of DNA, & the DNA is just okay quality. I did a clean up and ran them on the Miseq. Some of the sequencing quality is poor. Any recommendations for improving the data quality on the analysis side? Or even something to do if I redo the library prep? Thanks! The samples I prepped ran fine, so it isn’t a run issue.
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Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?
Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating...10-14-2026, 09:55 AM
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