We are planing to move to Solexa from 454, the problem is if it is possible to get a good assemble from only Solexa data for the human Mitochondrial DNA(17Kb, circular, no repetitive element)?
-
-
I have seen good assembly for plant mitochondrial DNA using 35bp reads. The fact that there's no repetitive element makes it easier. -
Do you have the reference paper?Comment
-
Oops...my mistake. What I saw is chloroplast DNA (~160kb) not mitochondrial DNA. I think it's not published yet.Comment
-
I asked some experts, it seems highly possible, although nobody publish it yet.
Comment
-
We have sequenced human mito DNA on the Illumina and get very good results.Comment
-
Hi Bruce,
What's your software to do the assembling?Comment
-
Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?
Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating...10-14-2026, 09:55 AM -
Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.
We asked six companies the same four questions to learn about their latest products. The new technologies bring a lot to the table, including rethinking sequencing...
Comment