MiSeq run failed (no usable signal found in the images)

[amrvx4]

I had a run on miseq using v3 kit and within an hour the run stopped with the error "no usable signal found in the images it is possible that clustering has failed". I have read that hardware could be an issue but I was just wondering can this happen with wrong library quantification? I did quantified library using Picogreen assay kit and diluted the libraries, however now I feel suspicious about the kit's accuracy.

So I have 2 questions:
1) is it possible that clustering failed that early if the library was not diluted correctly.

2) since clustering didn't even start, what would be the status of that flowcell? I do have an old expired v3 kit I wanted to try but do not have a flowcell for it. So my question is, can this flowcell be reused with another kit since no clustering was started?

Thanks

[hirohaha]

So how was it?
any update?

just happened to me
JUST HAPPENED, just yesterday
I am waiting for an explanation from the IlluminaTech

I quantified my sample with Qubit 1X HS DNA, I also checked with BioAnalyzer for size distribution. Everything was OK. I also re-checked the pooled concentration, it was expected, based on the average library size.

Mine was stopped just before two hours.
the error message was "ThroughFocusScan CoarseFocus failed to find focus"

[beancat]

can you attach some of the first few images from the cycles? I expect its overclustered and you can tell from that

[adx2021]

It is likely to be overclustering. We encountered similar problems before, need to re-quantified (qPCR) the libraries.

[hirohaha]

Illumina did not really give me an answer,
they (kind of) insisted that it was the library fault.
I re-run with a new cartridge (v3),
with the exact same library.
It was fine.