So I can't find the formulation/concentration of the Low TE that comes with the Ion kits anyone know what the concentration and pH of the Tris-EDTA should be?
-
-
Low TE generally is 10 mM Tris pH 8 0.1 mM EDTA, don't know for sure if Ion Torrent uses that. -
-
Hi there
Is this Low TE then acceptable to use for diluting gDNA in the Ion Shear fragment Xpress kit? I did an ethanol precipitation and re suspended the gDNA in RNase-free dH2O to be safe but I would prefer to use it as is, if it wont affect the reaction.
JuliJuli Hepple (MSc Genetics)Central analytical facilitiesDNA Sequencing UnitSouth AfricaComment
-
Yes, I would use this buffer to resuspend your DNAs.Comment
-
NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification, detection...Yesterday, 06:46 AM -
Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?
Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating...10-14-2026, 09:55 AM
Comment