Hello,
I am using the Ion Shear Plus reagents kit to prepare libraries for the PGM. I input 200 ng of DNA (measured by Qubit) but am only getting 0.01-0.1 ng/ul at the end of the process. This is enough to continue to sequencing, but I'm wondering if there's anything I can do to improve the yield slightly.
I am shearing the DNA for 7 minutes, which looks really good on the E-gel. It seems like maybe the amplification step is not working? I use zymo instead of the Ampure beads for cleanup and elute in 100 mM Tris.
Thanks,
Jessica
I am using the Ion Shear Plus reagents kit to prepare libraries for the PGM. I input 200 ng of DNA (measured by Qubit) but am only getting 0.01-0.1 ng/ul at the end of the process. This is enough to continue to sequencing, but I'm wondering if there's anything I can do to improve the yield slightly.
I am shearing the DNA for 7 minutes, which looks really good on the E-gel. It seems like maybe the amplification step is not working? I use zymo instead of the Ampure beads for cleanup and elute in 100 mM Tris.
Thanks,
Jessica
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