Hi guys,
I have a question regarding how to validate a completed bacterial genome. The sequencing technology used was the Illumina GAIIX, and the annotations were done in CLC bio.
I've recently finished the gap closing, and I've confirmed the alignment using CLCbio and ClustalOmega.
My supervisor insists that I validate the genome, but I have absolutely no clue how to do that. I've completely closed all the gaps (resulting in a final single fasta file output), and there are no longer any ambiguous nucleotides.
is there something I'm missing?
Thanks.
I have a question regarding how to validate a completed bacterial genome. The sequencing technology used was the Illumina GAIIX, and the annotations were done in CLC bio.
I've recently finished the gap closing, and I've confirmed the alignment using CLCbio and ClustalOmega.
My supervisor insists that I validate the genome, but I have absolutely no clue how to do that. I've completely closed all the gaps (resulting in a final single fasta file output), and there are no longer any ambiguous nucleotides.
is there something I'm missing?
Thanks.
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