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  • Flushing flow cells

    I was wondering if anyone has actually ever successfully flushed a flow cell. I have tried three or four times and it has always failed. Today, I started with a flow cell that had 1300 pores. I loaded a library. As it turns out, I had made a huge mistake, I made my library without using ligase in the ligation step (I promise this is the first time it happened in very many libraries). IN any case, after about 5 minutes into the run, I realized it was a mess and then also realized my mistake. I thought, no problem, I'll just flush the cell and reload a new library. I flushed the cell, waited 60 minutes, as is required, and checked the flow cell. It had only 580 pores available, so it was not possible to reload it. Really? Don't get me wrong, I love ONT, but I am just baffled at the flushing story....

  • #2
    You will always loose pores when flushing. Prepping and adding the newly made library wasn't an option?

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