I was wondering if anyone has actually ever successfully flushed a flow cell. I have tried three or four times and it has always failed. Today, I started with a flow cell that had 1300 pores. I loaded a library. As it turns out, I had made a huge mistake, I made my library without using ligase in the ligation step (I promise this is the first time it happened in very many libraries). IN any case, after about 5 minutes into the run, I realized it was a mess and then also realized my mistake. I thought, no problem, I'll just flush the cell and reload a new library. I flushed the cell, waited 60 minutes, as is required, and checked the flow cell. It had only 580 pores available, so it was not possible to reload it. Really? Don't get me wrong, I love ONT, but I am just baffled at the flushing story....
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NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification, detection...Yesterday, 06:46 AM -
Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?
Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating...10-14-2026, 09:55 AM
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