Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Flushing flow cells

    I was wondering if anyone has actually ever successfully flushed a flow cell. I have tried three or four times and it has always failed. Today, I started with a flow cell that had 1300 pores. I loaded a library. As it turns out, I had made a huge mistake, I made my library without using ligase in the ligation step (I promise this is the first time it happened in very many libraries). IN any case, after about 5 minutes into the run, I realized it was a mess and then also realized my mistake. I thought, no problem, I'll just flush the cell and reload a new library. I flushed the cell, waited 60 minutes, as is required, and checked the flow cell. It had only 580 pores available, so it was not possible to reload it. Really? Don't get me wrong, I love ONT, but I am just baffled at the flushing story....

  • #2
    You will always loose pores when flushing. Prepping and adding the newly made library wasn't an option?

    Comment

    Latest Articles

    Collapse

    • seqadmin
      The Impact of AI in Genomic Medicine
      by seqadmin



      Artificial intelligence (AI) has evolved from a futuristic vision to a mainstream technology, highlighted by the introduction of tools like OpenAI's ChatGPT and Google's Gemini. In recent years, AI has become increasingly integrated into the field of genomics. This integration has enabled new scientific discoveries while simultaneously raising important ethical questions1. Interviews with two researchers at the center of this intersection provide insightful perspectives into...
      02-26-2024, 02:07 PM
    • seqadmin
      Multiomics Techniques Advancing Disease Research
      by seqadmin


      New and advanced multiomics tools and technologies have opened new avenues of research and markedly enhanced various disciplines such as disease research and precision medicine1. The practice of merging diverse data from various ‘omes increasingly provides a more holistic understanding of biological systems. As Maddison Masaeli, Co-Founder and CEO at Deepcell, aptly noted, “You can't explain biology in its complex form with one modality.”

      A major leap in the field has
      ...
      02-08-2024, 06:33 AM

    ad_right_rmr

    Collapse

    News

    Collapse

    Topics Statistics Last Post
    Started by seqadmin, Today, 06:12 AM
    0 responses
    13 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 02-23-2024, 04:11 PM
    0 responses
    64 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 02-21-2024, 08:52 AM
    0 responses
    70 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 02-20-2024, 08:57 AM
    0 responses
    60 views
    0 likes
    Last Post seqadmin  
    Working...
    X