Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • metheuse
    Member
    • Jan 2013
    • 84

    #1

    Find large indels from PacBio reads?

    I know Quiver and GATK have been bundled in SMRT pipeline to call variants (the GATK module only finds SNPs though). I'm wondering if there are other tool(s) that can be used for this purpose, especially to find large indels. Thanks.
  • mchaisso
    Member
    • Apr 2008
    • 84

    #2
    I have an unsupported version of BLASR that does better alignment across large gaps. I have used this to simply count indel variants (really mobile element insertion) using PacBio reads. PM me if you want access to this; I've been hesitant to post it online since it is on a branch that is not updated with bug fixes, etc.. The unsupported version uses an affine alignment penalty. Without this, bases tend to "bleed" out into gaps and make it difficult to call exactly where a gap is.

    -mark

    Comment

    Latest Articles

    Collapse

    • SEQadmin2
      16S/ITS Profiling vs. Shotgun Metagenomics: What Changes in Your Workflow?
      by SEQadmin2




      NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification, detection...
      Today, 06:46 AM
    • SEQadmin2
      Multiomic Liquid Biopsy: What DNA/RNA Panels Reveal That DNA Alone Can’t
      by SEQadmin2



      Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?

      Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating
      ...
      10-14-2026, 09:55 AM

    ad_right_rmr

    Collapse

    News

    Collapse

    Topics Statistics Last Post
    Started by SEQadmin2, 10-28-2026, 09:57 AM
    0 responses
    22 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 10-28-2026, 09:51 AM
    0 responses
    14 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 10-22-2026, 11:13 AM
    0 responses
    68 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 10-22-2026, 10:48 AM
    0 responses
    48 views
    0 reactions
    Last Post SEQadmin2  
    Working...