DNA clean up for Pacbio

[suass]

Hi,

I'm planning a de novo sequencing on isolated gram positive bacteria.
I heard from the sequencing provider that this technique is really sensitive to contamination. Especially for gram positive bacteria, what I understand is the residue or sugar from cell wall easily cause problem in the library preparation.
The isolate is gram positive cellulose degrading bacteria, the situation might worse.
So I plan to grow it on cellobiose, but meanwhile, if there is a good way to remove polysaccharide after the DNA extraction?

Thanks in advance!

[han526]

DNA Normalizer can actually be used for polysaccharide removal. Just putting in a little more beads.

Hi,

I'm planning a de novo sequencing on isolated gram positive bacteria.
I heard from the sequencing provider that this technique is really sensitive to contamination. Especially for gram positive bacteria, what I understand is the residue or sugar from cell wall easily cause problem in the library preparation.
The isolate is gram positive cellulose degrading bacteria, the situation might worse.
So I plan to grow it on cellobiose, but meanwhile, if there is a good way to remove polysaccharide after the DNA extraction?

Thanks in advance!

[suass]

Finally I used the PowerClean DNA Clean-Up Kit from Mobio, and the sequencing works out fine.