This paper has done a comparison of various long range polymerases:http://www.nature.com/articles/srep05737
You can break your region of interest to smaller size with some overlap and amplify them. Pushing PCR to 40kb may require some tweaking and optimisation.
I think it is best to ask your candidate sequencing provider about the size of amplicon that they can sequence and gives best results in their hands. Sometimes achieving what is written on a specification sheet is difficult or just a random event.
You can break your region of interest to smaller size with some overlap and amplify them. Pushing PCR to 40kb may require some tweaking and optimisation.
I think it is best to ask your candidate sequencing provider about the size of amplicon that they can sequence and gives best results in their hands. Sometimes achieving what is written on a specification sheet is difficult or just a random event.
Comment