How should I compute the sampling depth or cells when I use pacbio RNA sequencing?
I want to do the RNA sequencing using pacbio SMRT, I have got the transcriptome information, but I don't know how many cells should I use ? And what the sampling depth is enough?
Thank for any adviceO(∩_∩)O~
Thank for any adviceO(∩_∩)O~
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happy -
It depends on your goal and the organism's clade, of course. Are you doing novel transcript discovery from a well-known organism, for example? And what kingdom is it in? -
Thanks for reply@Brian BushnelllO(∩_∩)O~ Yes. I want to do the novel transcript discovery and to accomplish one species's annotiation....happyComment
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