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  • Rui Guo
    Member
    • Apr 2016
    • 18

    #1

    Can I use velvet to assemble SMRT reads?

    I don't know whether it's possible to use velvet to assemble SMRT reads. Also I want to know how to know the read coverage of SMRT. Is that a function of the number of the SMRT cells? What other parameter affect the result?
  • GenoMax
    Senior Member
    • Feb 2008
    • 7142

    #2
    "Read coverage of SMRT" is not logical (unless you want a gross number e.g. total bases from SMRTcell/genome size).

    I assume you want to know the characteristics (number of reads, length, median size etc) of data from a SMRT cell? This should be available in the SMRTportal where SMRTcell data is processed. If a sequence provider did the analysis for you ask them for a copy of the report of basic analysis (e.g. RS_reads_of_insert).

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    • rhall
      Senior Member
      • Aug 2012
      • 324

      #3
      Velvet does not assemble SMRT data. Take a look at https://github.com/PacificBioscience...Bio-Long-Reads for a list of assemblers.

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