I read the paper Reducing assembly complexity of microbial genomes with single-molecule sequencing, and I find it use PacbioToCA to do self-correction. But I think PacbioToCA uses short reads(NGS reads) to do hybrid read correction, not self. And the paper says it uses PBcR and NGS reads to do hybrid read correction, and in the section PBcR correction pipeline in that paper, it mentioned it's feasible to use C2 reads to do self correction. I'm confused what method it really use to do self correction.
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http://canu.readthedocs.io/en/stable/ Is the latest implementation of a pipeline to assemble Pacbio only data based on Celera Assembler (CA).
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by SEQadmin2
NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification, detection...-
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by SEQadmin2
Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?
Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating...-
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10-14-2026, 09:55 AM -
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