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When my Pacbio sequencing was done I ended up with 18 subreads that each contained xxxx number of sequences (6,431,149 sequences in total for all 18 subreads). Due to particular reasons of having an allocation at a super computer I was having troubles running the Canu command that encompasses all three stages: correct, trim, assemble. So what I decided to do was individually execute the Canu-correct command on each of the 18 subreads. So now I have a list of 18 subreads that have been corrected. My questions is would there be a difference if I would have executed the Canu-correct command on one single file that was a concatenation of all 18 subreads? I ask because I am going to do some downstream analysis with the corrected reads and want to make sure that I am doing this right. Thank you!
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