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  • yaximik
    Senior Member
    • Apr 2011
    • 199

    SeqStudio users?

    I wonder if anyone has information whether SeqStudio cathode buffer is the same as other running /cathode buffers for earlier ABI CE sequencers. In other words, can 10x running buffers for ABI CE sequencers available on the market can be used as SeqStuido cathode buffer?
  • Markiyan
    Senior Member
    • Sep 2010
    • 126

    #2
    You can try it (especially with the used cartridge), but first I would measure the conductivity/pH of the ready cathode/anode buffer solution, than dillute the ABI CE buffer to the same ionic strength as new instrument ones. The buffering capacity of universal buffer is lower, than specifically made anode/cathode ones, but by how much, a test titration by acid/base can tell you.

    Comment

    • hamzairshad267
      Junior Member
      • Apr 2023
      • 5

      #3
      I am using ABI3500 for capillary fragment analysis, Can I just refill the cathode and anode buffer instead of changing them every time to make it cost effective?
      Thankyou

      Comment

      • ahs810
        Junior Member
        • May 2025
        • 2

        #4
        Hi there,
        Any one use the product of Nimagen (Shop - NimaGen.com | Innovators in DNA Sequencing Technologies - NimaGen), they offer buffer for Seqstudio, has anyone use?
        Last edited by ahs810; 05-20-2025, 11:33 PM.

        Comment

        • ahs810
          Junior Member
          • May 2025
          • 2

          #5
          Hi there,
          i have an issue, we purchased SeqStudio in 2021, problem is it often hang, 1st run in majority sizing issue (same sample in 2nd injection has data peaks & sized), contact support but no use. do anyone face the same issue?​

          Comment

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