IQR report
Hello
I performed a run for instrument qualification and troubleshooting (IQ run).
One flowcell (FC1) passed all the checklist, however, the the other flowcell (FC2) failed two of them.
From the panel error heatmap of the FC2, the error rate gradually increased from center to edge of the flowcell.
Is it a bead pouring problem or fluidic problem?
If there was an error with pouring beads, all of the 5 primer cylcels were supposed to show high error rate. However, everyting was OK until the 4th primer, and the error rate dramatically increased at the 5th primer cycles.
There is a summary below.
1. FC1 passed all, but FC2 did not.
2. The panel error spreads out from center to edge in FC2.
3. Among 5 primer cycles, only 5th cylce showed high error rate.
What would be the problem?
Thank you for any idea!!!
I performed a run for instrument qualification and troubleshooting (IQ run).
One flowcell (FC1) passed all the checklist, however, the the other flowcell (FC2) failed two of them.
From the panel error heatmap of the FC2, the error rate gradually increased from center to edge of the flowcell.
Is it a bead pouring problem or fluidic problem?
If there was an error with pouring beads, all of the 5 primer cylcels were supposed to show high error rate. However, everyting was OK until the 4th primer, and the error rate dramatically increased at the 5th primer cycles.
There is a summary below.
1. FC1 passed all, but FC2 did not.
2. The panel error spreads out from center to edge in FC2.
3. Among 5 primer cycles, only 5th cylce showed high error rate.
What would be the problem?
Thank you for any idea!!!
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