IQ run

[eoh001]

Hello

I am posting the cycle scans from an IQ run, and I posted the related thread 'IQR report' a few days ago.
From the IQ run report, the FC2 failed some of the check lists.
I want to know what was wrong with the FC2.
For detailes, refer to the previous thread 'IQR report'.
Please give me any idea about what happened in the FC2.

I have another question.
I analyzed the run log of the IQ run, and I found that it takes longer time to scan FC1 than FC2. Is it normal?

[asaleh]

what size is your slide for this run is this a quad? 200 mil usable beads is really low for a whole slide you should expect closer to 700 mil.

I have never done an IQ run though, our service engineer performed an IQ run when they installed the machine but since then I have not had to do one.

If this is for a whole slide I would say that it could be a deposition issue or a bead quant issue.