Hi all,
I'm doing a mate-paired library for SOLiD 5500.
When I run the E-Gel to confirm a 300 bp fragment, the result was 200 bp.
When I did the Nick translation reaction, the protocol suggests 10-11 min and I set 10,5 min. All incubations and stop reactions were very very strict, as the protocol says.
Can I use this library to continue the experiment?
Can someone help me?
Thanks,
Soledad.
I'm doing a mate-paired library for SOLiD 5500.
When I run the E-Gel to confirm a 300 bp fragment, the result was 200 bp.
When I did the Nick translation reaction, the protocol suggests 10-11 min and I set 10,5 min. All incubations and stop reactions were very very strict, as the protocol says.
Can I use this library to continue the experiment?
Can someone help me?
Thanks,
Soledad.
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