Hi all,
I'm working with gDNA from bacteria and from human, doing mate-paired libraries to sequence using SOLID 4.
I want to sequence these samples using a slide divided in 4 parts:
1/4 for bacterial DNA
3/4 for human DNA
I think I have to use E20 to perform emPCR. But I don't know if it is correct.
I'll have to prepare 3 volumes of E20 for human DNA and 1 volume of E20 for bacterial DNA?
Is this correct?
Please, I need your help!
Best regards,
Soledad
I'm working with gDNA from bacteria and from human, doing mate-paired libraries to sequence using SOLID 4.
I want to sequence these samples using a slide divided in 4 parts:
1/4 for bacterial DNA
3/4 for human DNA
I think I have to use E20 to perform emPCR. But I don't know if it is correct.
I'll have to prepare 3 volumes of E20 for human DNA and 1 volume of E20 for bacterial DNA?
Is this correct?
Please, I need your help!
Best regards,
Soledad
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