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  • primary analysis parameters

    Hello
    I have the run reports from 2 sequencing runs (FC1&2, mulitplexing paired-end). I wonder that the values of the primary analysis parameters in the 2 runs are good.

    1. Could give me any comments on the quality of the runs?
    2. For defult setting for the parameters, which values are general?

    ___________________________________________________________

    parameter ______|_______Flow cell 1_____|_____ Flow cell 2_______
    ________________Barcode|__F5__|__F3__| barcode|__F5__|__F3___
    ___________________________________________________________
    Panel alignment__| 97% | 97% | 97% | 99% | 99% | 99%

    HQ beads_______| 26% | 26% | 38% | 22% | 22% | 41%

    Bead retension___| 94% | 94% | 93% | 98% | 99% | 35%

    Color balance____| 14% | 14% | 12% | 7% | 13% | 12%

    QV median_______| 13 | 13 | 18 | 13 | 12 | 20

    Blur value________| 2.079 | 2.079 | 1.975 | 1.943 | 1.960 | 1.943

    Blur population____| 0% | 0% | 0% | 0% | 0% | 0%

    Exposure value____| 0.169 | 0.0576 | 0.0576 | 0.171 | 0.176 | 0.0686

    Exposure ________| 0% | 0% | 0% | 0% | 0% | 0%
    population

    Exposure time_____| 255 |211 | 255 | 250 | 208 | 250
    Last edited by eoh001; 11-16-2011, 04:11 AM.

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