Hello
I have the run reports from 2 sequencing runs (FC1&2, mulitplexing paired-end). I wonder that the values of the primary analysis parameters in the 2 runs are good.
1. Could give me any comments on the quality of the runs?
2. For defult setting for the parameters, which values are general?
___________________________________________________________
parameter ______|_______Flow cell 1_____|_____ Flow cell 2_______
________________Barcode|__F5__|__F3__| barcode|__F5__|__F3___
___________________________________________________________
Panel alignment__| 97% | 97% | 97% | 99% | 99% | 99%
HQ beads_______| 26% | 26% | 38% | 22% | 22% | 41%
Bead retension___| 94% | 94% | 93% | 98% | 99% | 35%
Color balance____| 14% | 14% | 12% | 7% | 13% | 12%
QV median_______| 13 | 13 | 18 | 13 | 12 | 20
Blur value________| 2.079 | 2.079 | 1.975 | 1.943 | 1.960 | 1.943
Blur population____| 0% | 0% | 0% | 0% | 0% | 0%
Exposure value____| 0.169 | 0.0576 | 0.0576 | 0.171 | 0.176 | 0.0686
Exposure ________| 0% | 0% | 0% | 0% | 0% | 0%
population
Exposure time_____| 255 |211 | 255 | 250 | 208 | 250
I have the run reports from 2 sequencing runs (FC1&2, mulitplexing paired-end). I wonder that the values of the primary analysis parameters in the 2 runs are good.
1. Could give me any comments on the quality of the runs?
2. For defult setting for the parameters, which values are general?
___________________________________________________________
parameter ______|_______Flow cell 1_____|_____ Flow cell 2_______
________________Barcode|__F5__|__F3__| barcode|__F5__|__F3___
___________________________________________________________
Panel alignment__| 97% | 97% | 97% | 99% | 99% | 99%
HQ beads_______| 26% | 26% | 38% | 22% | 22% | 41%
Bead retension___| 94% | 94% | 93% | 98% | 99% | 35%
Color balance____| 14% | 14% | 12% | 7% | 13% | 12%
QV median_______| 13 | 13 | 18 | 13 | 12 | 20
Blur value________| 2.079 | 2.079 | 1.975 | 1.943 | 1.960 | 1.943
Blur population____| 0% | 0% | 0% | 0% | 0% | 0%
Exposure value____| 0.169 | 0.0576 | 0.0576 | 0.171 | 0.176 | 0.0686
Exposure ________| 0% | 0% | 0% | 0% | 0% | 0%
population
Exposure time_____| 255 |211 | 255 | 250 | 208 | 250