Header Leaderboard Ad

Collapse

5500 Wildfire

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • #16
    Originally posted by Chipper View Post
    How many raw reads did you get per lane?
    450 million and 400 million Total reads from the multiplex report. I think this can be higher if we find the optimal amount to load on the slide.
    Originally posted by SeqTruth View Post
    Can anyone tell me if Wildfire will work with standard SOLiD libraries, or even better, what the Wildfire adapter sequences are?
    You can PCR the Wildfire adapters to a 5500 library so that you can run it on the wildfire. I don't know the sequence.

    Comment


    • #17
      Hi,

      I will be in AGBT looking at this from a different angle. Perhaps someone has link/more information please.

      I run a genomics facility for CR-UK and we have GA's, HiSeq and an Ion torrent to do validation sequencing and for that it's a good little tool. The down fall is the emulation PCR. Now when something goes wrong it is pretty much back to that. It’s expensive and time consuming.

      Now, if you haven't worked out what I want to do yet, I want to use the wildfire library production and its non-emulation PCR and run on my Ion torrent but I can't find any details. As academia I do not have to worry about the issues companies have with IP. So, I want to give it a go. Can anyone send me links to papers, information or help on this I will buy you a drink as AGBT.

      All the best

      Nik

      Comment


      • #18
        This doesn't sound practical to me, the amplification of the WF protocol happens on the slide (as with the Illumina range) so it won't be portable to the ion chip....could you imagine using the GA protocol to make libraries for the Ion?

        My understanding is that there will be an emulsionPCR free strategy on the ion, they are calling 'Avalanche' but I don't think there is a release data yet.

        Comment


        • #19
          Hi, I need your help please.

          I accidentaly ordered run cycle buffers and buffer D for solid wildfire, although we do have 5500xl only. Do you think i can use these buffers, or throw them away?
          thanks.

          Comment


          • #20
            Originally posted by Brunda View Post
            Hi, I need your help please.

            I accidentaly ordered run cycle buffers and buffer D for solid wildfire, although we do have 5500xl only. Do you think i can use these buffers, or throw them away?
            thanks.

            The actual sequencing on Wildfire is chemically the same as 5500xl provided they haven't moved the buffer locations on deck (that's easy to check).
            It should work.

            Comment


            • #21
              Got 650 million reads from a slide.

              Comment


              • #22
                Originally posted by JackQuist View Post
                The actual sequencing on Wildfire is chemically the same as 5500xl provided they haven't moved the buffer locations on deck (that's easy to check).
                It should work.
                Buffer D is different and even for those that are same, total volume in most of the bottles are different too since WF doesn't use that much buffer.

                Comment


                • #23
                  Originally posted by Zaag View Post
                  Got 650 million reads from a slide.
                  6 lanes total? The number doesn't sound right to me.

                  Comment


                  • #24
                    Originally posted by bioits View Post
                    6 lanes total? The number doesn't sound right to me.
                    It's a little lower than you might like but we've seen runs like this. It equates to around 150,000 reads per panel doesn't it?

                    Comment


                    • #25
                      Originally posted by bioits View Post
                      6 lanes total? The number doesn't sound right to me.
                      1 slide = 3 lanes

                      Comment


                      • #26
                        Ah sorry, getting my threads mixed up!

                        Comment

                        Latest Articles

                        Collapse

                        • seqadmin
                          Improved Targeted Sequencing: A Comprehensive Guide to Amplicon Sequencing
                          by seqadmin



                          Amplicon sequencing is a targeted approach that allows researchers to investigate specific regions of the genome. This technique is routinely used in applications such as variant identification, clinical research, and infectious disease surveillance. The amplicon sequencing process begins by designing primers that flank the regions of interest. The DNA sequences are then amplified through PCR (typically multiplex PCR) to produce amplicons complementary to the targets. RNA targets...
                          03-21-2023, 01:49 PM
                        • seqadmin
                          Targeted Sequencing: Choosing Between Hybridization Capture and Amplicon Sequencing
                          by seqadmin




                          Targeted sequencing is an effective way to sequence and analyze specific genomic regions of interest. This method enables researchers to focus their efforts on their desired targets, as opposed to other methods like whole genome sequencing that involve the sequencing of total DNA. Utilizing targeted sequencing is an attractive option for many researchers because it is often faster, more cost-effective, and only generates applicable data. While there are many approaches...
                          03-10-2023, 05:31 AM

                        ad_right_rmr

                        Collapse

                        News

                        Collapse

                        Topics Statistics Last Post
                        Started by seqadmin, 03-24-2023, 02:45 PM
                        0 responses
                        10 views
                        0 likes
                        Last Post seqadmin  
                        Started by seqadmin, 03-22-2023, 12:26 PM
                        0 responses
                        13 views
                        0 likes
                        Last Post seqadmin  
                        Started by seqadmin, 03-17-2023, 12:32 PM
                        0 responses
                        17 views
                        0 likes
                        Last Post seqadmin  
                        Started by seqadmin, 03-15-2023, 12:42 PM
                        0 responses
                        22 views
                        0 likes
                        Last Post seqadmin  
                        Working...
                        X