Dear forum members,
Thanks for this wonderful site and your ever-so-useful contributions.
I have a question about the mapping of short reads in the context of a metagenomic study:
What impact has sequencing read length (35b vs 50b for instance) on read mapping?
I'm thinking of these few aspects, but the list is is far from being exhaustive:
- mapping input: number of sequences generated
- mapping process: quality/speed of alignments
- mapping output: false positive hits, false negative hits...
Theoretical answers and experiences you have had are very welcome.
Thanks!
Thanks for this wonderful site and your ever-so-useful contributions.
I have a question about the mapping of short reads in the context of a metagenomic study:
What impact has sequencing read length (35b vs 50b for instance) on read mapping?
I'm thinking of these few aspects, but the list is is far from being exhaustive:
- mapping input: number of sequences generated
- mapping process: quality/speed of alignments
- mapping output: false positive hits, false negative hits...
Theoretical answers and experiences you have had are very welcome.
Thanks!
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