So you did a test run and it appeared to work, then you did a regular run and all your beads went to waste again....is that correct?
-
-
-
I think this goes back to what "snetmcom" said, if there are beads in the waste then they must have passed through the filter at some stage. They shouldn't be able to do this so it must be the source of your problem.
There are several washes of your beads on the filter, during one wash an aliqout is taken off to the QC output (before enrichment). This should happen even if your emPCR has failed. If you're not getting anything in the QC output then it looks like your beads are passing through the filter for some reason. Probably a good idea to check your lot numbers with your rep.
JPCComment
-
The immune system’s power comes from its genetic diversity, allowing myriad threats to be neutralized through first recognizing foreign antigens. That diversity is also what makes the immune system so difficult to study. Recent advances in sequencing technology and computational biology, however, are giving researchers new tools to understand immune responses and immune-related diseases in greater detail.
This convergence of genetics, immunology, and computation...Yesterday, 05:41 AM
Comment