Has anybody tried to optimise the protocol to improve the on-axis %?
I'm getting 55-65% on-axis beads, so still quite a bit of polyclonal.
We tried to lower the amount of library to go into the ePCR with some variable results (0.45pM at the moment and still trying to drop...)
A side problem - the beads stick to the lo-bind tubes a lot after the enrichment which is a pain...
I'm getting 55-65% on-axis beads, so still quite a bit of polyclonal.
We tried to lower the amount of library to go into the ePCR with some variable results (0.45pM at the moment and still trying to drop...)
A side problem - the beads stick to the lo-bind tubes a lot after the enrichment which is a pain...