Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • zhanxw
    Junior Member
    • Jun 2009
    • 7

    #1

    Can mapreads deal with paired end reads?

    mapreads documentation does not mention how to map paired end reads.
    Does anyone have any experience?
  • lmf_bill
    Member
    • Jul 2008
    • 36

    #2
    not, for paired-end reads, you can firstly do single-end reads mapping by "mapreads", then "pairing" can be used for pairing.

    Comment

    • zhanxw
      Junior Member
      • Jun 2009
      • 7

      #3
      do you know where to find "pairing" documentation?
      I found some options are not hard to understand, e.g. "flen", "rlen", "Z" (from pairing options below).
      I hope SOLiD gives more examples in the future....

      pairing(V2.5:06/09/2009) R3file F3file lower upper [M=mode][f=flen][r=rlen][Z=(L1,U1);(L2,U2)...][S=refseq] [O=0/1] [U=0/1] [T=##] [X=matrix] [F=maskingF] [R=maskingR] [G=0/1][I=##][D=##][i=##][d=##][P=0/1][q=##][V=tmp_res_file][z=maxrescue][a=TreshR][b=TreshF][B=##][E=##][u=snprate][H=iubmapping][A=VA][m=mis_p][L=min_len][h=histogramFile][x=dropscore][y=indelcutoff][o=outfile][np=#processors]
      M=mode (default 0): 0 R3-F3 pairing; 1: F5-F3 pairing
      O=0 sorted order; O=1 pairing by order(1st w 1st, 2nd w/ 2nd & so on)
      U=0 all non-rescued pairs (default), U=1 only unique non-rescued pair
      UR=0 all rescued pairs (default), UR=1 only unique rescued pair (default)
      Uh=0 for histograms, include all pairs, Uh=1 only include unique pairs in histogramT=## is the cutoff for rescue output
      P=0/1 i/o format 0 pos.mis, 1: chrom_pos.mis
      q= number of rescued alignments
      B,E specify the beginning and ending penal numbers;
      A=0/1 whether treat Valid adjacent as 1
      H=0/1 whether allow IUB mapping

      Comment

      • JDS
        Junior Member
        • Sep 2009
        • 4

        #4
        http://solidsoftwaretools.com, you can download the corona_lite software package ( mapreads included ) and the documentation.
        you can find the pairing part, as well as the SNP and small indel.

        Comment

        • zhanxw
          Junior Member
          • Jun 2009
          • 7

          #5
          I found the documentation! Thanks, JDS.

          Comment

          Latest Articles

          Collapse

          • SEQadmin2
            16S/ITS Profiling vs. Shotgun Metagenomics: What Changes in Your Workflow?
            by SEQadmin2




            NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification, detection...
            Today, 06:46 AM
          • SEQadmin2
            Multiomic Liquid Biopsy: What DNA/RNA Panels Reveal That DNA Alone Can’t
            by SEQadmin2



            Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?

            Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating
            ...
            10-14-2026, 09:55 AM

          ad_right_rmr

          Collapse

          News

          Collapse

          Topics Statistics Last Post
          Started by SEQadmin2, 10-28-2026, 09:57 AM
          0 responses
          22 views
          0 reactions
          Last Post SEQadmin2  
          Started by SEQadmin2, 10-28-2026, 09:51 AM
          0 responses
          14 views
          0 reactions
          Last Post SEQadmin2  
          Started by SEQadmin2, 10-22-2026, 11:13 AM
          0 responses
          67 views
          0 reactions
          Last Post SEQadmin2  
          Started by SEQadmin2, 10-22-2026, 10:48 AM
          0 responses
          47 views
          0 reactions
          Last Post SEQadmin2  
          Working...