Yield from line of SOLID 5500 chip?

[Krosh]

Hi everyone!

Could you post your statistics of read number from one line SOLID 5500 chip?
Our result are in range 90-160 mln per line. But maximal yield must be 200 mln. (90Gb/6/75 = 200 mln)

And how can I increase number of immobilized microbeads on chip?

Thank you.

[JPC]

Hi Krosh, with a panel density of 220,000 and 708 readable panels a single lane will give you 155,760,000 reads so I think your range sounds normal. Where are you getting your 90Gb figure from? is it marketing material? if so I would ignore it.

Getting a higher bead density on the chip seems to be a simple case of putting more beads in the flowcell and ensuring your all of your bead prep steps are done well.

JPC