Yes I have successfully used lifescope for mouse genome small RNA mapping. What version of miRBase were you trying to incorporate? We never updated ours after setting it up for miRBasev18.
If you are still having troubles I could send you the mouse input files we successfully used? In the meantime I'll find some time to update our system to miRBasev19 to see if it works on our end.
Cheers
Seqanswers Leaderboard Ad
Collapse
Announcement
Collapse
No announcement yet.
X
-
Lifescope smallRNA module with mouse genome
Has anybody had any luck in running in smallRNA module for Lifescope for mouse genome. ?
I've been using the input files I downloaded from mirbase (miRNA.txt for mature, mmu.gff2/mmu.gff3 for precursor) . Lifescope fails with the following error.
secondary-mm9-smallrnamapping [2%] [JOB_FAILURE] Error in generating miRBase invocation command. Pipeline Exception Could not find any precursors in the genome reference file. Input GFF file /data/LifeScopeDisk/referenceData/external/mm9/mirbase/mmu.gff and the input reference file /data/LifeScopeDisk...............Tags: None
Latest Articles
Collapse
-
by seqadmin
The complexity of cancer is clearly demonstrated in the diverse ecosystem of the tumor microenvironment (TME). The TME is made up of numerous cell types and its development begins with the changes that happen during oncogenesis. “Genomic mutations, copy number changes, epigenetic alterations, and alternative gene expression occur to varying degrees within the affected tumor cells,” explained Andrea O’Hara, Ph.D., Strategic Technical Specialist at Azenta. “As...-
Channel: Articles
07-08-2024, 03:19 PM -
ad_right_rmr
Collapse
News
Collapse
Topics | Statistics | Last Post | ||
---|---|---|---|---|
Started by seqadmin, Yesterday, 06:46 AM
|
0 responses
9 views
0 likes
|
Last Post
by seqadmin
Yesterday, 06:46 AM
|
||
Started by seqadmin, 07-24-2024, 11:09 AM
|
0 responses
26 views
0 likes
|
Last Post
by seqadmin
07-24-2024, 11:09 AM
|
||
Started by seqadmin, 07-19-2024, 07:20 AM
|
0 responses
159 views
0 likes
|
Last Post
by seqadmin
07-19-2024, 07:20 AM
|
||
Started by seqadmin, 07-16-2024, 05:49 AM
|
0 responses
127 views
0 likes
|
Last Post
by seqadmin
07-16-2024, 05:49 AM
|
Leave a comment: