Does anybody have SOLiD v. 3.5 run protocol

[seqbren]

Hello, everyone.

I have already discribed our problem on this board. In short: we currently operate SOLiD 4, but still have a considerable amount of version 3.5 consumables, which we would really like to use.

The only difference between these two versions is the run protocol. It is a xml file (named something like SOLID3Opti.xml or similar) located by default in c:\protocols\ folder of Windows operated computer. It refers to fluidic subsystems script files (they have '.waa' extension) which are located by default in c:\flowcellscripts\ folder.

If anybody could provide me the content of these two folders we could use our v. 3.5. consumables. Thank you in advance.

[snetmcom]

you can run 3.5 chemistry on a solid4 without these changes. you just needed to add this : SOLiD™ Mixing Strip Tubes with Zinc.

[seqbren]

Thank you, snetmcom.

Have you personally tried this approach? For I was sure that SOLiD™ Mixing Strip Tubes with Zinc is only required for ver. 4 sequencing reagents, while ver 3.5 is self-sufficient.

I have managed to find the fluidics scipts, still in search for .xml run protocol ver 3.5 file.