Hi all,
I am preparing whole transcriptome library for SOLiD 5500. Can anybody tell me the typical yield of the amplified library. What should be the titration point for starting the ePCR step. ?? The protocol recommends a range between 0.4-0.8pM.
Thanks,
Regards,
Jaya
I am preparing whole transcriptome library for SOLiD 5500. Can anybody tell me the typical yield of the amplified library. What should be the titration point for starting the ePCR step. ?? The protocol recommends a range between 0.4-0.8pM.
Thanks,
Regards,
Jaya